Technologies for Spatial Bioengineering

​Image-based interrogation of molecular distributions have been widely used in biology, but with limited number of markers due to the color limitations of the microscopes. However, cellular decisions are guided by interactions of at least 20,000 protein-coding genes and their downstream and upstream protein factors. This large number of molecular maps has been studied by single-cell RNA sequencing or mass spectrometer, but the spatial details of positions of cells in complex tissues have been lost during the measurements.

Our research team aims to bridge this gap by developing multiplex image barcoding to visualize and quantify many transcripts, proteins, and metabolites at the molecular scale.

Our team develops purpose-driven multiplex bioimaging technologies that can visualize two/three-dimensional (2D/3D) spatial heterogeneity of biological systems at the single cell and subcellular level.

SPATIAL GENOMICS: Sequential FISH and Correlation FISH

SPATIAL PROTEOMICS: SUPER-RESOLUTION CODEX

SPATIAL METABOLOMICS: ION BEAM TOMOGRAPHY